TB-500 (Thymosin Beta-4)
The Regenerative Peptide for Healing, Inflammation, and Tissue Repair
What is TB-500 (Thymosin Beta-4)?
TB-500 is a synthetic peptide fragment derived from Thymosin Beta-4 (Tβ4), a naturally occurring protein found in nearly all human and animal cells. TB-500 is a potent actin-binding peptide that plays a central role in tissue repair, cellular migration, angiogenesis, inflammation modulation, and regeneration.
While full-length Thymosin Beta-4 is composed of 43 amino acids, TB-500 is a shorter, bioactive region (typically the 17 amino acid segment responsible for its regenerative effects) and is favored for its greater stability, cost-effectiveness, and ease of synthesis in research protocols.
TB-500 has become a cornerstone in regenerative and recovery research, frequently studied for applications in wound healing, soft tissue recovery, organ repair, chronic inflammation, and even cardiac tissue regeneration.
How Does TB-500 Work?
TB-500’s primary biological function is the regulation of cell-building protein actin, a vital component in the cytoskeleton of all eukaryotic cells. By sequestering G-actin monomers and facilitating actin polymerization, TB-500 enables cell migration, proliferation, and structural repair.
Mechanism of Action:
- Binds and modulates G-actin, enhancing cytoskeletal dynamics
- Promotes angiogenesis (new blood vessel formation)
- Regulates inflammatory cytokine expression
- Stimulates tissue-specific stem cell migration and differentiation
- Facilitates collagen deposition and ECM (extracellular matrix) repair
- Enhances cell survival under hypoxic and oxidative stress conditions
This multifaceted activity positions TB-500 as a master regulator of healing—able to influence cellular behavior across multiple tissue types.
Benefits of TB-500 in Research
🧬 Tissue Repair and Regeneration
- Accelerates wound closure and scar remodeling
- Promotes healing in muscle, tendon, ligament, and cartilage tissue
- Enhances regeneration in nerve and epithelial tissues
❤️ Cardiac and Vascular Health
- Improves cardiac muscle remodeling post-ischemic injury (e.g., heart attack)
- Reduces fibrosis and promotes angiogenesis in heart tissue
- Protects endothelial cells from oxidative damage and atherosclerosis
🛡️ Anti-Inflammatory and Immune Modulation
- Downregulates pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)
- Promotes M2 macrophage polarization (anti-inflammatory phenotype)
- Supports immune tolerance and reduces autoimmune overactivity
🐾 Veterinary and Sports Research Applications
- Used in equine medicine to treat tendon/ligament injuries and lameness
- Studied in athletes for faster recovery, reduced scarring, and muscle regeneration
- Shows promise in models of arthritis, corneal injury, and gut mucosa repair
TB-500 vs BPC-157 vs Thymosin Alpha-1
| Feature | TB-500 | BPC-157 | Thymosin Alpha-1 |
|---|---|---|---|
| Primary Function | Actin regulation & repair | Gastrointestinal & tendon healing | Immune modulation |
| Inflammatory Modulation | Strong | Moderate | Strong |
| Angiogenesis Support | Strong | Moderate | Weak |
| Ideal Use Case | Muscle, soft tissue, organs | GI tract, ligaments | Viral immunity, cancer adjunct |
| Stack Potential | High | High | Moderate |
TB-500 is considered the most broad-spectrum regenerative peptide among its class.
Suggested Dosage & Research Protocols
Subcutaneous or Intramuscular Injection:
- Loading phase: 2.0–2.5mg twice weekly for 4–6 weeks
- Maintenance phase: 1.0–2.0mg once every 7–10 days
- Injection site should be near the area of injury for localized action
Research Notes:
- Can be safely combined with BPC-157 for synergistic tissue-specific healing
- Cycles often span 4–12 weeks, followed by evaluation or tapering
Safety Profile of TB-500
TB-500 has been shown to be non-toxic and well tolerated in animal and cell line models. It does not exhibit carcinogenic or mutagenic properties and has not shown suppression of endogenous hormone production.
Common Observations:
- Faster post-injury recovery
- Improved flexibility and reduced stiffness
- Enhanced wound closure rates
Rare or Possible Side Effects:
- Mild fatigue or lightheadedness (typically at high doses)
- Temporary increase in local inflammation (part of the healing cascade)
- No reported immunogenicity in studies
Reference Studies:
- Xing, Y et al., “Progress on the Function and Application of Thymosin β4.” Front Endocrinol, 2021. https://pmc.ncbi.nlm.nih.gov/articles/PMC8724243/
- Safer D. “Thymosin beta 4 and Fx, an actin-sequestering peptide, are indistinguishable.” J Bio Chem, 1991. https://pubmed.ncbi.nlm.nih.gov/1999398/
Storage Instructions
- Store lyophilized TB-500 at 2–8°C
- Reconstitute with bacteriostatic water or sterile saline
- Refrigerate post-reconstitution and use within 14–30 days
TB-500 Stack Recommendations for Advanced Regenerative Protocols
TB-500 is often included in comprehensive healing and performance stacks:
- BPC-157 – powerful dual action on collagen synthesis, inflammation, and healing kinetics
- GHK-Cu – supports angiogenesis, hair regrowth, and anti-fibrotic skin repair
- ARA-290 – combines anti-inflammatory action with erythropoietic and neural protection
- NAD+ or SS-31 – for mitochondrial support during tissue remodeling
Is TB-500 Right for Your Research?
If your research targets injury repair, fibrosis reduction, soft tissue regeneration, neuroregeneration, or angiogenesis, TB-500 offers a broad, effective, and biologically active approach to enhancing recovery. It is especially powerful when tissue damage involves muscle, tendon, ligament, or cardiovascular structures.
Whether you are investigating sports injury recovery, chronic inflammation pathways, or age-related tissue degeneration, TB-500 is a proven performer in both preclinical and translational research models.
Final Thoughts
TB-500 (Thymosin Beta-4) is a cornerstone regenerative peptide with unmatched scope in tissue healing, inflammation control, and vascular support. With a rich history in both clinical and performance-based applications, it offers one of the most versatile and well-tolerated platforms for advanced recovery and repair protocols.
Lab of RAD offers high-purity TB-500 peptide for research applications in musculoskeletal recovery, soft tissue healing, and cellular repair across a wide range of biological systems.
⚠️ FDA Disclaimer
This content is provided for educational and research purposes only. TB-500 has not been evaluated or approved by the U.S. Food and Drug Administration (FDA). It is not intended to diagnose, treat, cure, or prevent any disease.
Lab of RAD peptides are for laboratory research use only and are not for human consumption.